There is no abstract for this very informative tutorial, but Dr. Tyagarjan is graciously allowing the posting of the entire contents on the site, following the meeting.

Quantitative protein expression profiling by Isotope-coded Affinity Tag (ICAT>TM) labeling is an exciting new alternative to similar experiments using 2-D gel technology. This technique is much more amenable to automation than 2-D gel analysis and imaging and offers the advantages of  looking at membrane proteins as well as low abundance proteins. However, this technology has its own limitations such as being cysteine specific. In addition, high quality quantitation (C.V s less than 20%) from LC/MS/MS data, where the peptide pairs do not entirely coelute, has its challenges. This talk will cover an overview of ICAT technology and focus on several issues involved with processing this data, and its implications on experimental design.